Coding

Part:BBa_K1903006:Design

Designed by: Sofía Vieto Fonseca   Group: iGEM16_TEC-Costa_Rica   (2016-10-14)


Split TEV N-IntN DnaE Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 544


Design Notes

This part is a device created with the split TEV N terminal-Intein N terminal DnaE K1903005 fused to the Lacl regulated promoter R0010 assembled with the RBS Elowitz BBa_B0034.

We assembled this device and verified it in an agarose gel electrophoresis:



We were able to express the split TEV N terminal-Intein N terminal DnaE in the following SDS-PAGE:


Source

References